Cultured Human Retinal Pigment Epithelial (hRPE) Sheets: A Search for Suitable Storage Conditions

Bidirectional glutathione transport by cultured human retinal pigment epithelial cells.

PURPOSE To characterize glutathione (GSH) transport by cultured human retinal pigment epithelial (HRPE) cells. METHODS Cultured HRPE cells were pretreated with acivicin for GSH efflux and with buthionine sulfoximine for GSH uptake to prevent the breakdown and resynthesis of GSH. Efflux was measured by the linear rate of accumulation of GSH in the supernatant; uptake was measured using [35S] G...

Oxidant-induced apoptosis in cultured human retinal pigment epithelial cells.

PURPOSE To determine the mechanism of oxidant-induced cell death in cultured human retinal pigment epithelium (hRPE). METHODS Cultured hRPE cells were treated with different concentrations of a chemical oxidant, t-butylhydroperoxide (tBH), for different periods of time. Apoptosis was determined with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and flow c...

Single-channel recordings from cultured human retinal pigment epithelial cells

We have applied patch-clamp techniques to on-cell and excised-membrane patches from human retinal pigment epithelial cells in tissue culture. Single-channel currents from at least four ion channel types were observed: three or more potassium-selective channels with single-channel slope conductances near 100, 45, and 25 pS as measured in on-cell patches with physiological saline in the pipette, ...

Effects of bioflavonoids on cultured human retinal pigment epithelial cells

Adrenomedullin in cultured human retinal pigment epithelial cells.

PURPOSE To determine whether adrenomedullin (ADM), a vasorelaxant peptide is produced and secreted by human retinal pigment epithelial (RPE) cells, whether ADM expression is regulated by inflammatory cytokines and a growth factor, and whether ADM has proliferative effects on these cells. METHODS Production and secretion of ADM by cultured human RPE cells were examined by Northern blot analysi...